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Publications from CUBE

CUBE is a core facility of the UNIK - Food, Fitness & Pharma initiative.

Follow the links below to read the full text and summaries of the scientific papers published by the CUBE's scientists.

Original papers

New lead compounds for the effect of whey protein hydrolysate revealed by LC-MS metabolomics. Stanstrup, J., Rasmussen, J. E., Ritz, C., Holmer-Jensen, J., Hermansen, K., and Dragsted,L.O. Metabolomics 1-18 online 10.1007/s11306-013-0607-9(2014)(accepted) 

Whey protein delays gastric emptying and suppresses plasma fatty acids and their metabolites compared to casein, gluten and cod protein. Stanstrup,J., Schou,S.S., Holmer-Jensen,J., Hermansen,K., Dragsted, L.O. J. Proteome Res. (online doi 10.1021/pr401214w).(2014) Whey protein has been demonstrated to improve fasting lipid and insulin response in overweight and obese individuals. To establish new hypotheses for this effect and to investigate the impact of stomach emptying, we compared plasma profiles after intake of whey isolate (WI), casein, gluten (GLU), and cod (COD).

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Metabolite profiling and beyond: approaches for the rapid processing and annotation of human blood serum mass spectrometry data. Stanstrup J, Gerlich M, Dragsted LO, Neumann S. Anal Bioanal Chem. 2013 Jun.
In this paper, we describe data processing and metabolite identification approaches which lead to a rapid and semi-automated interpretation of metabolomics experiments. Data from metabolite fingerprinting using LC-ESI-Q-TOF/MS were processed with several open-source software packages, including XCMS and CAMERA to detect features and group features into compound spectra...

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UPLC-ESI-QTOF/MS and multivariate data analysis for blood plasma and serum metabolomics: effect of experimental artefacts and anticoagulant. Barri T, Dragsted LO. Anal Chim Acta. 2013 Mar.
Clotting and anticoagulation of blood samples may give rise to different metabolic profiles of serum and plasma samples, respectively. The anticoagulant used for blood plasma preparation may affect the resulting metabolic profile due to different mechanisms involved in anticoagulation by various agents, e.g. heparin, EDTA and citrate...

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Molecular structure of large-scale extracted β-glucan from barley and oat: Identification of a significantly changed block structure in a high β-glucan barley mutant. Mikkelsen MS, Jespersen BM, Larsen FH, Blennow A, Engelsen SB. Food Chem. 2013 Jan.
Health effects of β-glucan are typically related to dose, size and viscosity without taking the specific molecular structure into account. High β-glucan mutant barley, mother barley and oat β-glucans were large-scale extracted by comparable protocols using hot water, enzyme assisted hydrolysis and ethanol precipitation leading to similar molecular masses (200-300kDa)...

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Metabolic fingerprinting of high-fat plasma samples processed by centrifugation- and filtration-based protein precipitation delineates significant differences in metabolite information coverage. Barri T, Holmer-Jensen J, Hermansen K, Dragsted LO. Anal Chim Acta. 2012 Mar.
Metabolomics and metabolic fingerprinting are being extensively employed for improved understanding of biological changes induced by endogenous or exogenous factors. Blood serum or plasma samples are often employed for metabolomics studies. Plasma protein precipitation (PPP) is currently performed in most laboratories before LC-MS analysis...

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